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This is in sharp contrast with a high sulfur content observed in many B. subtilis products like mucopeptides, spore germination inhibitors and antibiotics. Sulfur metabolism in B. subtilis is initiated by homocysteine as an intermediate in methionine biosynthesis [@bib5]. HmgA is a cysteine desulfurase which converts homocysteine to cystathionine and Hcy into S-Adenosyl-[L]{.smallcaps}-methionine (SAM), the universal methyl donor. Hcy was not detected in *B. subtilis* [@bib5], [@bib17]. The nucleotide content of the DNA in B. subtilis has been reported as G/(A+G) ratio at 0.5-0.6 with a rather low G content of 0.27-0.37, whereas the G/(A+G) ratio in *C. crescentus* is 0.73 [@bib18], [@bib19]. The increased G content in *B. subtilis* genome in our study may be one of the reasons why this organism is recalcitrant to produce sulfur-rich compounds. The genome content of sulfur-rich *B. amyloliquefaciens* and *B. subtilis* strain NCIB 3610/C9 is not only low but also provides evidence for slow sulfur metabolism in the organism [@bib12], [@bib13]. We gratefully acknowledge the financial support of the Department of Biotechnology, Govt. of India, through the BTIS programme and partial funding through ITC-PEPSU. ![Coupled GC--MS of the supernatant from *Bacillus* sp. strain F425 in DSMZ medium supplemented with 0.25% ferrous sulfate and 0.1% glucose.](gr1){#f0005} System Requirements: Microsoft Windows 10 64 bit or Windows 8.1 64 bit 1 Gb of Ram 2Gb of free hard drive space DirectX 11 Multi-core CPU VS 2012 IDE A 64 bit Windows development machine will be needed for the build. A typical development machine may consist of the following: Processor: Intel Core i5-3570K 4.4 Ghz Memory: 16 Gb RAM Graphics: NVIDIA GeForce GTX 660 Hard Drive: 6 Gb Free Space
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